Proteomic analysis of purified Newcastle disease virus particles

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Proteomic analysis of chicken peripheral blood mononuclear cells after infection by Newcastle disease virus

Characteristic clinical manifestations of Newcastle disease include leukopenia and immunosuppression. Peripheral blood mononuclear cells (PBMCs) are the main targets of Newcastle disease virus (NDV) infection. To survey changes in proteomic expression in chicken PBMCs following NDV infection, PBMC proteins from 30 chickens were separated using two- dimensional electrophoresis (2-DE) and subject...

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Replication of Newcastle Disease Virus

Infection of L cells with Newcastle disease virus (NDV) results in abortive replication, with the production of very few plaque-forming units (PFU) of virus per cell (W. C. Wilcox, Virology 9:30, 1959; K. Paucker et al., Virology 17:301, 1962). It is known that interferon is synthesized by L cells infected with NDV, and the possibility exists that this endogenously produced inhibitor may be res...

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Newcastle disease (ND) is a major threat to poultry industry production throughout developing countries. The Newcastle disease viruses (NDVs) infecting industrialized and indigenous poultry in Iran are velogenic strains and responsible for the frequent outbreaks of ND in poultry farms even in vaccinated flocks causing serious economic losses in the commercial and indigenous poultry. However, va...

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Studies on Newcastle Disease Virus

1. It is likely that certain tailed and filamentous particles seen on electron microscope examination of partially purified saline suspensions of Newcastle virus are the individual virus particles because: (a) They have a highly characteristic shape not seen in other virus preparations. (b) They are present whenever the virus is present in high concentration. (c) Their size agrees with the size...

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Structural analysis of peptides that interact with Newcastle disease virus.

A peptide with the sequence CTLTTKLYC has previously been identified to inhibit the propagation of Newcastle disease virus (NDV) in embryonated chicken eggs and tissue culture. NDV has been classified into two main groups: the velogenic group, and mesogenic with lentogenic strains as the other group based on its dissociation constants. In this study the peptide, CTLTTKLYC, displayed on the pIII...

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ژورنال

عنوان ژورنال: Proteome Science

سال: 2012

ISSN: 1477-5956

DOI: 10.1186/1477-5956-10-32